Sample type: Serum

Test name: Amikacin

Condition / Indication: Requesters have found these assays to be of value in all patients treated for >48 hours

Advance warning: Not usually required during our core working hours (Mon – Fri 09:00 – 17:15) or Sat 09:00-12:00 for this assay. Please contact the laboratory to discuss the possibility of this assay being performed on Sundays and Bank Holidays.

Sample required: Approx. 1-2mL of separated serum (the minimum acceptable is 150µL). Samples may be sent by post. Transport time must be <7 days. Samples should be kept at 4°c if there is a delay in sending. Please contact laboratory if in doubt as sample integrity may be affected. Samples must be sealed and packed to conform to current regulations. Leaking samples may not be processed.

Timing of samples: We recommend a pre dose sample and a post dose sample taken 1h after end of IV/IM admin.

Test information required: • Patient name, sex, date of birth
• Laboratory number
• Dosage, frequency and timing of samples; especially important with once-daily dosing
• Clinical Summary
• Address for report
• Phone number for report or email address for an electronic copy
• Contact name
• Appropriate hazard warnings

Results: Results will be telephone or emailed on the day of receipt for samples received between 9 am and 3 pm Monday to Friday and by 10 am on a Saturday. A written confirmation report will be sent by post.

Reference range: Once Daily Pre dose <5mg/L, Post dose 40-45mg/L. BD or TDS Pre <10mg/L Post 20-30mg/L

Container: Serum (Gold top)

Referred outside NBT for analysis? No

Discipline: Antimicrobial Reference Laboratory

Turnaround time: <1 day

Re-assay interval: 6-8 Days OD, 3-7 days BD/TDS – Assuming initial results are within expected range.