Sample type: Serum

Test name: ANCA

Condition / Indication: Two major vasculitis associated patterns - 1)C-ANCA (cytoplasmic, usually anti-PR3 positive) associated with Granulomatosis with polyangiitis (Wegener's) (>80%), Microscopic Polyangiitis, Eosinophilic Granulomatosis with polyangiitis (Churg Strauss).  2)P-ANCA (perinuclear, often anti-MPO positive) seen in Granulomatosis with polyangiitis (Wegener's), Microscopic Polyangiitis, Granulomatosis with polyangiitis (Churg Strauss). N.B. P-ANCA (anti-MPO positive or negative) also seen in CTD (e.g.rheumatoid, SLE) with or without vasculitis, in some infections or may be drug induced..

Special precautions & notes: Store at 2-80C prior to referral.

Reference range: ANCA – reported as a Immunofluorescence pattern
PR3 <2.0 IU/ml (2.0 – 3.0 IU/ml = equivocal)
MPO <3.5 IU/ml ( 3.5-5.0 IU/ml = equivocal)

Container: Serum (Gold top)

Ideal volume (mL): 2 mL

Referred outside NBT for analysis? No

Discipline: Immunology

Section: Autoimmunity

Turnaround time: 4 working days

Method:

ANCA – Immunofluorescence
PR3/MPO – FEIA (Fluorescence enzyme immunoassay)

Further information: EQA: UK NEQAS ANCA/GBM Antibodies